Proteomics Facility

Sample submissions

2D Gel Electrophoresis

Please fill out a single form for each sample being submitted for analysis by 2D SDS-PAGE. Note that a separate form is required for sequencing by mass spectrometry. After sample analysis is completed, an Interdepartmental Transfer (IDT) form will be sent to your billing contact by the Business Administrator for the Department of Biochemistry and Molecular Biology.

Instructions:

• Investigator Information: Provide information about the person submitting the sample. Fill out as indicated.
• Billing Information: Provide information as indicated about the person in your department who handles billing. This is the person to whom your IDT will be sent after sample analysis is completed.
• Payment Information: Fill out as indicated. Some of this information is required for billing purposes, and some is needed for core facility record keeping. The authorized signature, if possible, should be for the principal investigator of the laboratory submitting the sample. WE WILL NOT PERFORM SAMPLE ANALYSIS WITHOUT AN AUTHORIZED SIGNATURE.
• Sample information: All of this information is extremely important for obtaining the best results possible for your sample. Please fill it out completely.
  • Sample buffer: Please note that sample buffers should not contain SDS and should not have a total salt concentration greater than 40 mM. The investigator will be responsible for payment for unsuccessful results obtained as a result of samples submitted in inappropriate buffers.
  • Approximate amount of protein: Please indicate the estimated concentration of your protein in ug/mL. The recommended lower limit is 350 ug/mL.
  • IPG Strip pH range: Choose the range that is appropriate for your sample based on its pI. Unless otherwise indicated, a broad range (3-10) strip will be used.
  • SDS-PAGE gel percentage: Choose a gel that is appropriate for obtaining optimal separation for your protein mixture. Unless otherwise indicated, an 8-16% Tris-HCl linear gradient gel will be used.
• Services requested: Place a check mark in the Service column for desired sample analysis. IF you wish for your gel to be stained with something other than SYPRO Ruby, please indicate what stain you want in the Special Instructions box.
  • Image analysis involves comparison between two gels using software that quantitates change in protein expression levels between two samples.
  • Spot picking involves the excision of proteins from 2D gels for sequencing via mass spectrometry. The ProPic robot is used for spot picking. NOTE: The decision about which spots to choose for analysis will be made by the investigator submitting the sample and not by Core Facility personnel.

Please click here for the 2D Gel Sample Submission Form
NOTE: You will need Adobe Acrobat Reader to view the above file. Click here to get it.

Mass Spectrometry

Please fill out a single form for each gel or sample being submitted for analysis by mass spectrometry. Note that a separate form is required for analysis by 2D gel electrophoresis. After sample analysis is completed, an Interdepartmental Transfer (IDT) form will be sent to your billing contact by the Business Administrator for the Department of Biochemistry and Molecular Biology.

Instructions:

• Investigator Information: Provide information about the person submitting the sample. Fill out as indicated.
• Billing Information: Provide information as indicated about the person in your department who handles billing. This is the person to whom your IDT will be sent after sample analysis is completed.
• Payment Information: Fill out as indicated. Some of this information is required for billing purposes, and some is needed for core facility record keeping. The authorized signature, if possible, should be for the principal investigator of the laboratory submitting the sample. WE WILL NOT PERFORM SAMPLE ANALYSIS WITHOUT AN AUTHORIZED SIGNATURE.
• Sample Information: Sample origin and species are helpful in making protein assignments based on database searching results, as is sample origin. Knowledge of sample buffer is important because not all buffers are compatible with electrospray ionization. This is especially important if you are providing your sample in solution rather than in a gel. If you are providing a gel, please indicate what the gel is stored in. Known and possible modifications (i.e. glycosylation, phosphorylation) are also very important in obtaining accurate results from database searching.
• Services Requested: For enzymatic digestion, the default enzyme is trypsin. If digestion with another proteolytic enzyme is desired, please indicate the enzyme requested in special instructions. Investigators may elect to perform their own data analysis. If this is the case, indicate none in the data analysis column and, under special instructions, indicate whether you want the raw data file or as a processed peak list file (this is probably what you want; database searching is generally carried out with a .pkl file).

Please click here for the Mass Spectrometry Sample Submission Form
NOTE: You will need Adobe Acrobat Reader to view the above file. Click here to get it.

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